Publications
The amino acid sequence of Emu osteocalcin: gas phase sequencing of Gla-containing proteins
Abstract
Osteocalcin (OC), the major gamma carboxyglutamic acid (Gla)-containing protein of vertebrate bone, has been isolated from bones of the emu (Dromaius novaehollandae) and the primary structure determined by a combination of gas phase N-terminal sequencing of the intact molecule and a proteolytic fragment, and carboxypeptidase Y C-terminal sequencing. Gla residues were located by counting tritium radioactivity in fractions from the N-terminal sequencing of the tritiated/thermally decarboxylated molecule. Emu OC consists of 48 amino acid residues containing 3 Gla residues, and a single disulphide bond. The C-terminal 29 residues are identical to those of the human and sheep OC sequences. Alignment of the N-terminal sequence against those of other OCs reveals greater sequence homology with chicken OC than with mammalian OCs.
Type | Journal |
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ISBN | 0158-5231 (Print) |
Authors | Huq, N. L.;Tseng, A.;Chapman, G. E. : |
Publisher Name | Biochem Int |
Published Date | 1987-01-01 |
Published Volume | 15 |
Published Issue | 2 |
Published Pages | 271-7 |
Status | Published in-print |
URL link to publisher's version | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=3501719 |