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Target-Specific Profiling of RNA m(5)C Methylation Level Using Amplicon Sequencing


Mapping the position and quantifying the level of 5-methylcytosine (m(5)C) as a modification in different types of cellular RNA is an important objective in the field of epitranscriptomics. Bisulfite conversion has long been the gold standard for the detection of m(5)C in DNA, but it can also be applied to RNA. Here, we detail methods for bisulfite treatment of RNA, locus-specific PCR amplification, and detection of candidate sites by sequencing on the Illumina MiSeq platform.

Type Journal
ISBN 1940-6029 (Electronic) 1064-3745 (Linking)
Authors Sibbritt, T.; Schumann, U.; Shafik, A.; Guarnacci, M.; Clark, S. J.; Preiss, T.
Publisher Name Methods in Molecular Biology
Published Date 2022-06-30
Published Volume 2404
Published Pages 375-392
Status Published in-print
DOI 10.1007/978-1-0716-1851-6_21
URL link to publisher's version