Publications
A non-genetic, cell cycle-dependent mechanism of platinum resistance in lung adenocarcinoma
Abstract
We previously used a pulse-based in vitro assay to unveil targetable signalling pathways associated with innate cisplatin resistance in lung adenocarcinoma (Hastings et al., 2020). Here, we advanced this model system and identified a non-genetic mechanism of resistance that drives recovery and regrowth in a subset of cells. Using RNAseq and a suite of biosensors to track single-cell fates both in vitro and in vivo, we identified that early S phase cells have a greater ability to maintain proliferative capacity, which correlated with reduced DNA damage over multiple generations. In contrast, cells in G1, late S or those treated with PARP/RAD51 inhibitors, maintained higher levels of DNA damage and underwent prolonged S/G2 phase arrest and senescence. Combined with our previous work, these data indicate that there is a non-genetic mechanism of resistance in human lung adenocarcinoma that is dependent on the cell cycle stage at the time of cisplatin exposure.
Type | Journal |
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ISBN | 2050-084X (Electronic) 2050-084X (Linking) |
Authors | Gonzalez Rajal, A.; Marzec, K. A.; McCloy, R. A.; Nobis, M.; Chin, V.; Hastings, J. F.; Lai, K.; Kennerson, M.; Hughes, W. E.; Vaghjiani, V.; Timpson, P.; Cain, J. E.; Watkins, D. N.; Croucher, D. R.; Burgess, A. |
Responsible Garvan Author | Associate Professor David Croucher |
Publisher Name | eLife |
Published Date | 2021-05-31 |
Published Volume | 10 |
Status | Published in-print |
DOI | 10.7554/eLife.65234 |
URL link to publisher's version | https://www.ncbi.nlm.nih.gov/pubmed/33983115 |