Publications
Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish
Abstract
Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells. Furthermore, we apply the TS to reveal biologically relevant changes in VE-cadherin tension that occur as the dorsal aorta matures and upon genetic and chemical perturbations during embryonic development.
| Type | Journal |
|---|---|
| ISBN | 2041-1723 (Electronic) 2041-1723 (Linking) |
| Authors | Lagendijk, A. K.; Gomez, G. A.; Baek, S.; Hesselson, D.; Hughes, W. E.; Paterson, S.; Conway, D. E.; Belting, H. G.; Affolter, M.; Smith, K. A.; Schwartz, M. A.; Yap, A. S.; Hogan, B. M. |
| Responsible Garvan Author | (missing name) |
| Publisher Name | Nature Communications |
| Published Date | 2017-11-10 |
| Published Volume | 8 |
| Published Issue | 1 |
| Published Pages | 1402 |
| Status | Published in-print |
| DOI | 10.1038/s41467-017-01325-6 |
| URL link to publisher's version | https://www.ncbi.nlm.nih.gov/pubmed/29123087 |
| OpenAccess link to author's accepted manuscript version | https://publications.gimr.garvan.org.au/open-access/14357 |