Publications

Publications Search

Search for publications by author
Search for publications by abstract keyword(s)

HENMT1 and piRNA Stability Are Required for Adult Male Germ Cell Transposon Repression and to Define the Spermatogenic Program in the Mouse

Abstract

piRNAs are critical for transposable element (TE) repression and germ cell survival during the early phases of spermatogenesis, however, their role in adult germ cells and the relative importance of piRNA methylation is poorly defined in mammals. Using a mouse model of HEN methyltransferase 1 (HENMT1) loss-of-function, RNA-Seq and a range of RNA assays we show that HENMT1 is required for the 2' O-methylation of mammalian piRNAs. HENMT1 loss leads to piRNA instability, reduced piRNA bulk and length, and ultimately male sterility characterized by a germ cell arrest at the elongating germ cell phase of spermatogenesis. HENMT1 loss-of-function, and the concomitant loss of piRNAs, resulted in TE de-repression in adult meiotic and haploid germ cells, and the precocious, and selective, expression of many haploid-transcripts in meiotic cells. Precocious expression was associated with a more active chromatin state in meiotic cells, elevated levels of DNA damage and a catastrophic deregulation of the haploid germ cell gene expression. Collectively these results define a critical role for HENMT1 and piRNAs in the maintenance of TE repression in adult germ cells and setting the spermatogenic program.

Type Journal
ISBN 1553-7404 (Electronic) 1553-7390 (Linking)
Authors Lim, S. L.; Qu, Z. P.; Kortschak, R. D.; Lawrence, D. M.; Geoghegan, J.; Hempfling, A. L.; Bergmann, M.; Goodnow, C. C.; Ormandy, C. J.; Wong, L.; Mann, J.; Scott, H. S.; Jamsai, D.; Adelson, D. L.; O'Bryan, M. K.;
Publisher Name PLOS GENET
Published Date 2015-01-01
Published Volume 11
Published Issue 10
Published Pages e1005620
Status Published in-print
URL link to publisher's version http://www.ncbi.nlm.nih.gov/pubmed/26496356
OpenAccess link to author's accepted manuscript version https://publications.gimr.garvan.org.au/open-access/13029