Publications
Reconfiguration of nucleosome depleted regions at distal regulatory elements accompanies DNA methylation of enhancers and insulators in cancer
Abstract
It is well established that cancer-associated epigenetic repression occurs concomitant with CpG island hypermethylation and loss of nucleosomes at promoters, but the role of nucleosome occupancy and epigenetic reprogramming at distal regulatory elements in cancer is still poorly understood. Here, we evaluate the scope of global epigenetic alterations at enhancers and insulator elements in prostate and breast cancer cells using simultaneous genome-wide mapping of DNA methylation and nucleosome occupancy (NOMe-seq). We find that the genomic location of nucleosome depleted regions (NDRs) is mostly cell type specific and preferentially found at enhancers in normal cells. In cancer cells however, we observe a global reconfiguration of NDRs at distal regulatory elements coupled with a substantial reorganization of the cancer methylome. Aberrant acquisition of nucleosomes at enhancer-associated NDRs is associated with hypermethylation and epigenetic silencing marks and conversely, loss of nucleosomes with demethylation and epigenetic activation. Remarkably, we show that nucleosomes remain strongly organized and phased at many facultative distal regulatory elements, even in the absence of a NDR as an anchor. Finally we find that key transcription factor (TF) binding sites also show extensive peripheral nucleosome phasing, suggesting the potential for TFs to organize NDRs genome-wide and contribute to deregulation of cancer epigenomes. Together our findings suggests that 'decommissioning' of NDRs and TFs at distal regulatory elements in cancer cells is accompanied by DNA hypermethylation susceptibility of enhancers and insulator elements which in turn may contribute to an altered genome-wide architecture and epigenetic deregulation in malignancy.
Type | Journal |
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Authors | Taberlay, P. C.; Statham, A. L.; Kelly, T. K.; Clark, S. J.; Jones, P. A.; |
Responsible Garvan Author | (missing name) |
Publisher Name | GENOME RESEARCH |
Published Date | 2014-12-01 |
Published Volume | 24 |
Published Pages | 1421-32 |
Status | Published in-print |
URL link to publisher's version | http://www.ncbi.nlm.nih.gov/pubmed/24916973 |
OpenAccess link to author's accepted manuscript version | https://publications.gimr.garvan.org.au/open-access/12223 |