Publications
Mapping of mitochondrial RNA-protein interactions by digital RNase footprinting
Abstract
Human mitochondrial DNA is transcribed as long polycistronic transcripts that encompass each strand of the genome and are processed subsequently into mature mRNAs, tRNAs, and rRNAs, necessitating widespread posttranscriptional regulation. Here, we establish methods for massively parallel sequencing and analyses of RNase-accessible regions of human mitochondrial RNA and thereby identify specific regions within mitochondrial transcripts that are bound by proteins. This approach provides a range of insights into the contribution of RNA-binding proteins to the regulation of mitochondrial gene expression.
Type | Journal |
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Authors | Liu, G.; Mercer, T. R.; Shearwood, A. M.; Siira, S. J.; Hibbs, M. E.; Mattick, J. S.; Rackham, O.; Filipovska, A. |
Publisher Name | Cell Reports |
Published Date | 2013-11-14 |
Published Volume | 5 |
Published Issue | 3 |
Published Pages | 839-48 |
Status | Published in-print |
DOI | 10.1016/j.celrep.2013.09.036 |
URL link to publisher's version | http://www.ncbi.nlm.nih.gov/pubmed/24183674 |
OpenAccess link to author's accepted manuscript version | https://publications.gimr.garvan.org.au/open-access/11926 |