Publications
Generation of human single domain antibody repertoires by kunkel mutagenesis
Abstract
Human antibody single domains are a promising new class of antibody fragments. Here we describe methods for the cloning of human V(H) and V(L) genes into phage and phagemid vectors. Furthermore, we provide detailed protocols for the generation of single domain antibody libraries by Kunkel mutagenesis and the analysis of diversity by DNA sequencing and superantigen binding.
| Type | Journal |
|---|---|
| ISBN | 1940-6029 (Electronic) 1064-3745 (Linking) |
| Authors | Rouet, R.; Dudgeon, K.; Christ, D.; |
| Responsible Garvan Author | Professor Daniel Christ |
| Publisher Name | Methods in Molecular Biology |
| Published Date | 2012-01-01 |
| Published Volume | 907 |
| Published Pages | 195-209 |
| Status | Published in-print |
| URL link to publisher's version | http://www.ncbi.nlm.nih.gov/pubmed/22907352 |
| OpenAccess link to author's accepted manuscript version | https://publications.gimr.garvan.org.au/open-access/11673 |