Publications
Single-cell RNA counting at allele and isoform resolution using Smart-seq3
Abstract
Large-scale sequencing of RNA from individual cells can reveal patterns of gene, isoform and allelic expression across cell types and states(1). However, current short-read single-cell RNA-sequencing methods have limited ability to count RNAs at allele and isoform resolution, and long-read sequencing techniques lack the depth required for large-scale applications across cells(2,3). Here we introduce Smart-seq3, which combines full-length transcriptome coverage with a 5' unique molecular identifier RNA counting strategy that enables in silico reconstruction of thousands of RNA molecules per cell. Of the counted and reconstructed molecules, 60% could be directly assigned to allelic origin and 30-50% to specific isoforms, and we identified substantial differences in isoform usage in different mouse strains and human cell types. Smart-seq3 greatly increased sensitivity compared to Smart-seq2, typically detecting thousands more transcripts per cell. We expect that Smart-seq3 will enable large-scale characterization of cell types and states across tissues and organisms.
Type | Journal |
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ISBN | 1546-1696 (Electronic) 1087-0156 (Linking) |
Authors | Hagemann-Jensen, M.; Ziegenhain, C.; Chen, P.; Ramskold, D.; Hendriks, G. J.; Larsson, A. J. M.; Faridani, O. R.; Sandberg, R. |
Responsible Garvan Author | Omid Faridani |
Publisher Name | NATURE BIOTECHNOLOGY |
Published Date | 2020-06-30 |
Published Volume | 38 |
Published Issue | 6 |
Published Pages | 708-714 |
Status | Published in-print |
DOI | 10.1038/s41587-020-0497-0 |
URL link to publisher's version | https://www.ncbi.nlm.nih.gov/pubmed/32518404 |