Publications
Human Antibody Bispecifics through Phage Display Selection
Abstract
We developed a repertoire approach to generate human antibody bispecifics. Using phage display selection of antibody heavy chains in the presence of a competitor light chain and providing a cognate light chain with an affinity handle, we identified mutations that prevent heavy/light chain mispairing. The strategy allows for the selection of human antibody chains that autonomously assemble into bispecifics.
| Type | Journal |
|---|---|
| ISBN | 1520-4995 (Electronic) 0006-2960 (Linking) |
| Authors | Luthra, A.; Langley, D. B.; Schofield, P.; Jackson, J.; Abdelatti, M.; Rouet, R.; Nevoltris, D.; Mazigi, O.; Crossett, B.; Christie, M.; Christ, D. |
| Responsible Garvan Author | Professor Daniel Christ |
| Publisher Name | Biochemistry |
| Published Date | 2019-04-02 |
| Published Volume | 58 |
| Published Issue | 13 |
| Published Pages | 1701-1704 |
| Status | Published in-print |
| DOI | 10.1021/acs.biochem.9b00037 |
| URL link to publisher's version | https://www.ncbi.nlm.nih.gov/pubmed/30900875 |